This is a list of papers related to PLA2s that have been published by the group. This list includes papers published together with collaborators.
PLA2 REVIEWS PUBLISHED:
Mapping structural determinants of biological activities in snake venom phospholipases A2 by sequence analysis and site directed mutagenesis. CHIOATO, L.; WARD, R.J. (2003) Toxicon 42: 869-883. (PDF file)
At the interface: crystal structures of Phospholipases A2. WARD, R. J.; AZEVEDO JR., W.F.; ARNI, R. K. (1998) Toxicon, 36(11):1623-1633. (PDF file)
Phospholipase A2 - A Structural Review. ARNI, R.K.; WARD, R. J. (1996) Toxicon, 34(8):827-841. (PDF file)
PLA2 PAPERS PUBLISHED (2000-2005):
A Predominant Role for Hydrogen Bonding in the Stability of the Homodimer of Bothropstoxin-I, a Lysine 49-Phospholipase A2. RULLER, R.; ARAGÃO, E. A.; CHIOATO, L.; LOPES FERREIRA, T.; DE OLIVEIRA, A.H.C.; SÀ, J.M.; WARD, R. J. (2005). Biochimie. (PDF file)
Structural insights for fatty acid binding in a Lys49-phospholipaseA2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid. WATANABE, L; SOARES, A.M.; WARD, R. J.; FONTES, M.R.M.; ARNI, R.K. (2005). Biochimie 87(2):161-167. (PDF file)
A Molecular Mechanism for Lys49-Phospholipase A2 activity based on ligand-induced conformational change. AMBROSIO, A.L.B.; NONATO, M.C.; SELISTRE DE ARAÚJO, H.S'; ARNI, R.K.; WARD, R. J.; OWNBY, C.L.; DE SOUZA, D.H.F.; GARRATT, R.C. (2005). J. Biol. Chem. 280:7326-7335. (PDF file)
Effect of Crotapotin on the Biological Activity of Asp49 and Lys49 Phospholipases A2 from Bothrops Snake Venoms. CECCHINI, A.L., SOARES, A.M. CECCHINI, R., WARD, R. J., GIGLIO, J.R. ARANTES, E.C. (2004) Comp. Biochem. and Physiol., C138:429-436. (PDF file)
The topology of the substrate binding site of a Lys49-PLA2 influences Ca2+-independent membrane damaging activity. SÁ, J.M., CHIOATO, L., FERREIRA, T.L.; de OLIVEIRA, A.H.C.; RULLER, R.; ROSA, J. C.; GREENE, L. J.; WARD, R. J. (2004) Biochem. J., 382:191-198. (PDF file)
Structural and functional analysis of BmjMIP, a phospholipase A2 myotoxin inhibitor protein from Bothrops moojeni snake plasma. SOARES, A.M.; MARCUSSI, S.; STÁBELI, R.G.; FRANÇA, S.C.; GIGLIO, J.R.; WARD, R. J., ARANTES, E.C. (2003) Biochem. Biophys. Res. Comm., 302:193-200. (PDF file)
Chemical denaturation of a homodimeric lysine-49 phospholipase A2: a stable dimer interface and a native monomeric intermediate. RULLER, R.; FERREIRA, T.L.; de OLIVEIRA, A.H.C.; WARD, R. J. (2003) Arch. Biochem. Biophys., 411:112-120. (PDF file)
Distinct sites for myotoxic and membrane-damaging activities in the C-terminal region of a Lys49-phospholipase A2. CHIOATO, L., de OLIVEIRA, A.H.C., RULLER, R., SÁ, J.M., WARD, R. J. (2002) Biochem. J., 366:971-976. (PDF file)
Active-site mutagenesis of a Lys49-phospholipase A2: biological and membrane-disrupting activities in the absence of catalysis. WARD, R. J.; CHIOATO, L.; de OLIVEIRA, A.H.C.; RULLER, R.; SÁ, J.M. (2002) Biochem. J., 362:89-96. (PDF file)
The effect of resonance energy homotransfer on the intrinsic tryptophan fluorescence emission of the Bothropstoxin-I dimer. de OLIVEIRA, A.H.C.; GIGLIO, J.R.; ANDRIÃO-ESCARSO, S.H., WARD, R. J. (2001) Biochem. Biophys. Res. Comm., 284:1011-1015. (PDF file)
A pH-induced dissociation of the dimeric form of a lysine 49-phospholipase A2 abolishes Ca2+-independent membrane damaging activity. de OLIVEIRA, A.H.C.; GIGLIO, J.R; ANDRIÃO-ESCARSO, S.H.; ITO, A.S.; WARD, R. J. (2001) Biochemistry, 40:6912-6920. (PDF file)
Dissociation of enzymatic and pharmacological properties of Piratoxins-I and III, two myotoxic phospholipases A2 from Bothrops pirajai snake venom. SOARES, A.M.; ANDRIÃO-ESCARSO, S.H., BORTOLETO, R.K.; RODRIGUES-SIMIONI, L.; ARNI, R.K.; WARD, R. J.; GUTIÉRREZ, J.M.; GIGLIO, J.R. (2001) Arch. Biochem. Biophys., 387(2):188-196. (PDF file)
Refolding and purification of Bothropstoxin-I, a Lys49-phospholipase A2 homologue, expressed as inclusion bodies in Escherichia coli. WARD, R. J.; de OLIVEIRA, A.H.C.; BORTOLETO, R.K.; ROSA, J.C.; FAÇA, V.M.; GREENE, L.J. (2001) Prot. Expr. Purif., 21:134-140. (PDF file)
PLA2 PAPERS PUBLISHED (prior to 2000):
Crystal structure of Myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani. ARNI, R.K.; FONTES, M.R.M.; BARBERATO, C.; GUTIÉRREZ, J.M.; DÍAZ, C.; WARD, R. J.. (1999) Arch. Biochem. Biophys., 366(2):177-182. (PDF file)
Structure of a Lys49-phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper). de AZEVEDO JR., W.F.; WARD, R. J.; GUTIÉRREZ, J.M.; ARNI, R.K. (1999) Toxicon, 37:371-384. (PDF file)
Crystallization and preliminary x-ray analysis of SIII-SPIII, a phospholipase A2 isolated from the venom of Bothrops jararacussu. de AZEVEDO JR., W.F.; FONTES, M.R.M.; WARD, R. J.; LOMBARDI, F.R.; CINTRA, A.C.O.; GIGLIO, J.R.; ARNI, R.K. (1998) Prot. Peptide Letters, 5(3):181-184. (PDF file)
Amino acid sequence of a myotoxic Lys49-phospholipase A2 homologue from the venom of Cerrophidion (Bothrops) godmani. de SOUSA, M.V.; MORHY, L.; ARNI, R.K.; WARD, R. J.; DÍAZ, C.; GUTIÉRREZ, J.M. (1998) Biochem. Biophys. Acta, 1384:204-208. (PDF file)
Crystal structure of Piratoxin-I: a calcium-independent, myotoxic phospholipase A2-homologue from Bothrops pirajai venom. de AZEVEDO JR., W.F.; WARD, R. J.; CANDURI, F., SOARES, A.; GIGLIO, J.R.; ARNI, R.K. (1998) Toxicon, 36(10):1395-1406. (PDF file)
Crystallization and preliminary x-ray diffraction analysis of a Lys49-PLA2 homologue from Cerrophidion godmani venom. de AZEVEDO JR., W.F.; WARD, R. J.; GUTIÉRREZ, J.M.; DÍAZ-OREIRO, C.; ARNI, R.K. (1998) Prot. Peptide Letters, 5(2):121-126. (PDF file)
Crystallization of Piratoxin I, a myotoxic Lys49-phospholipase A2 homologue isolated from the venom of Bothrops pirajai. CANDURI, F.; MANCUSO, L.C.; SOARES, A.M.; GIGLIO, J.R.; WARD, R. J.; ARNI, R.K. (1998) Toxicon, 36(3):547-551. (PDF file)
A SequenceSpace analysis of Lys49 phospholipases A2: clues towards identification of residues involved in a novel mechanism of membrane damage and in myotoxicity. WARD, R. J.; RODRIGUES ALVES, A.; RUGGIERO NETO, J.; ARNI, R.K.; CASARI, G. (1998) Protein Engineering, 11(4):285-294. (PDF file)
Crystallographic and spectroscopic characterization of a molecular hinge: conformational changes in Bothropstoxin I, a dimeric Lys49-Phospholipase A2 Homologue. GIOTTO, M.T.S.; GARRATT, R.C., OLIVA, G.; MASCARENHAS, Y.P.; GIGLIO, J.R.; CINTRA, A.C.O.; AZEVEDO JR., W.F.; ARNI, R.K.; WARD, R. J. (1998) Prot. Struct. Funct. Genet., 30:442-454. (PDF file)
Crystal structure of Myotoxin-II: a myotoxic phospholipase A2 homologue from Bothrops moojeni venom. de AZEVEDO JR., W. F.; WARD, R. J.; LOMBARDI, F.R.; GIGLIO, J.R.; SOARES, A.M.; FONTES, M.R.M.; ARNI, R.K. (1997) Prot. Peptide Letters 4(5):329-334. (PDF file)
Crystallization of Bothropstoxin II isolated from the venom of Bothrops jararacussu. BORTOLETO, R.K.; WARD, R. J..; GIGLIO, J.R.; CINTRA, A.C.O.; ARNI, R.K. (1996) Toxicon, 34(5):614-617. (PDF file)
Sequence of a cDNA encoding Bothropstoxin I, a myotoxin from the venom of Bothrops jararacussu. WARD, R. J.; MONESI, N.; ARNI, R.K.; LARSON, R.E.; PAÇÓ-LARSON, M.L. (1995) Gene, 156:305-306. (PDF file)
Crystallization and preliminary diffraction data of Bothropstoxin I isolated from the venom of Bothrops jararacusssu. ARNI, R.K.; WARD, R. J.; CINTRA, A.C.O.; GIGLIO, J.R. (1995) Toxicon, 33(3):383-386. (PDF file)
Structure of a calcium-independent phospholipase-like myotoxic protein from Bothrops asper venom. ARNI, R.K.; WARD, R. J.; GUTIERREZ, J.M.; TULINSKY, A. (1995) Acta. Cryst., D51:311-317. (PDF file)