Articles by RJW published between 1999-2002.
Structure
of a Lys49-phospholipase A2 homologue isolated from the venom of Bothrops
nummifer (jumping viper). de AZEVEDO JR., W.F.; WARD,
R.J.; GUTIÉRREZ, J.M.; ARNI, R.K. (1999) Toxicon,
37:371-384.
(Pdf file).
Three-dimensional
structure of ribonuclease T1 complexed with an isosteric phosphonate
substrate analogue of GpU: alternate substrate binding modes and catalysis.
ARNI, R.K.; WATANABE, L.; WARD, R.J.;
KREITMAN, R.J.; KUMAR, K.; WALZ JR., F.G. (1999) Biochemistry, 38(8):2452-2461.
(Pdf file).
Crystal
structure of Myotoxin II, a monomeric Lys49-phospholipase A2
homologue isolated from the venom of Cerrophidion
(Bothrops) godmani. ARNI, R.K.;
FONTES, M.R.M.; BARBERATO, C.; GUTIÉRREZ, J.M.; DÍAZ, C.; WARD, R.J. (1999) Arch. Biochem.
Biophys., 366(2):177-182.
(Pdf file).
The
amino acid sequence of ribitol Dehydrogenase-F, a mutant enzyme with improved
xylitol dehydrogenase activity. HOMSI-BRANDENBURGO, M.I.; TOYAMA, M.H.;
MARANGONI, S.; WARD, R.J.; GIGLIO,
J.R.; HARTLEY, B.S. (1999) J. Prot. Chem., 18(4):489-495.
(Pdf file).
A
stability transition at mildly acidic pH in the alpha-hemolysin (alpha-toxin)
from Staphylococcus aureus. BORTOLETO,
R.K.; WARD, R.J. (1999) FEBS
Lett., 459:438-442.
(Pdf file).
Refolding and purification
of Bothropstoxin-I, a Lys49-phospholipase A2 homologue, expressed as
inclusion bodies in Escherichia coli. WARD, R.J.; de
OLIVEIRA, A.H.C.; BORTOLETO, R.K.; ROSA, J.C.; FAÇA, V.M.; GREENE, L.J. (2001) Prot.
Expr. Purif., 21:134-140.
(Pdf file).
Dissociation
of enzymatic and pharmacological properties of Piratoxins-I and III, two
myotoxic phospholipases A2 from Bothrops
pirajai snake venom. SOARES, A.M.; ANDRIÃO-ESCARSO, S.H., BORTOLETO, R.K.;
RODRIGUES-SIMIONI, L.; ARNI, R.K.; WARD,
R. J.; GUTIÉRREZ, J.M.; GIGLIO, J.R. (2001) Arch. Biochem. Biophys., 387(2):188-196.
(Pdf file).
A
pH-induced dissociation of the dimeric form of a lysine 49-phospholipase A2
abolishes Ca2+-independent membrane damaging activity. de OLIVEIRA, A.H.C.; GIGLIO, J.R; ANDRIÃO-ESCARSO,
S.H.; ITO, A.S.; WARD, R.J. (2001) Biochemistry,
40:6912-6920.
(Pdf file).
The effect of resonance energy homotransfer on the
intrinsic tryptophan fluorescence emission of the Bothropstoxin-I dimer. de OLIVEIRA, A.H.C.; GIGLIO, J.R.; ANDRIÃO-ESCARSO,
S.H., WARD, R.J. (2001) Biochem. Biophys. Res. Comm.,
284:1011-1015.
(Pdf file).
Active-site
mutagenesis of a Lys49-phospholipase A2: biological and
membrane-disrupting activities in the absence of catalysis. WARD,
R.J.; CHIOATO, L.; de OLIVEIRA, A.H.C.; RULLER, R.; SÁ, J.M. (2002) Biochem.
J., 362:89-96.
(Pdf
file).
Distinct
sites for myotoxic and membrane-damaging activities in the C-terminal region of
a Lys49-phospholipase A2. CHIOATO, L., de OLIVEIRA, A.H.C., RULLER, R., SÁ, J.M., WARD,
R.J. (2002) Biochem. J., 366:971-976.
(Pdf file).
Intersexual
variations in the venom of the Brazilian "armed" spider Phoneutria
nigriventer (Keyserling, 1891). HERZIG, V., WARD, R.J., FERREIRA DOS SANTOS, W. (2002) Toxicon, 40:1399-1406.
(Pdf file).
Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin. KONOZY, E.H.E.; MULAY, R.; FAÇA, V.M.; WARD, R.J.; GREENE, L.J.; ROQUE-BARRIERA, M.C.; SABHARWAL, S.; BHIDE, S.V. (2002) Biochimie, 84:1035-1043. (Pdf file).